Tank sowing on the flora and an antibioticogram in gynecology are used to detect nonspecific microflora in the material taken from the genital tract, determine the potential pathogen of inflammation and its sensitivity to antibacterial drugs.
The study is performed using nutrient special media. Sowing on the flora is carried out in favorable conditions for the development of microorganisms. Next, the results are evaluated. Thus, the identification of microorganisms and the study of their biological and biochemical properties.
Modeling of the chemotherapeutic effect of drugs outside the woman’s body is carried out using special tests that determine the sensitivity of the selected microorganisms to bacteriophages, antiseptics and antibiotics. Thus, sowing on the flora allows you to choose an effective medication for each specific case.
The analysis, having high specificity, has, at the same time, its drawbacks. Due to the long term of the study, the increased requirements for the material collection process and the qualifications of laboratory staff, the duration of treatment increases. In clinical gynecology, sowing on the flora is used as an additional analysis in a complex of laboratory diagnostic measures.
Indications for the study are inflammation in the pelvic organs in women, including chronic or relapsing course, suspected sexually transmitted diseases. Also, sowing on the flora is necessary with a comprehensive examination in order to establish the causes of miscarriage or infertility.
Patients are sampled before the start of anti-inflammatory therapy and menstruation. Before taking the analysis, for fourteen days, any antibacterial drugs are stopped. At least one day you can not use vaginal suppositories, douche, and also have sexual contact. On the eve of the study, the passage of an ultrasound using a vaginal sensor is not recommended. For one or two hours, you should refrain from urinating, immediately before taking the analysis, hygiene procedures on the external genitalia should not be performed.
The test material should not contain blood impurities. Its capture must be done before manual gynecological examination.
The biological material is taken by a gynecologist when examined by vaginal mirrors, observing the rules of antiseptics. As a rule, the analysis is taken from the vaginal fornix (posterior), urethra and cervical canal. Before you take a smear from the cervical canal, the cervical flora is sterilized with saline. The mucous plug is previously removed . Biomaterial samples are taken using sterile cotton swabs, then they are placed in a sterile container. Labeled material is quickly sent to the bacteriological laboratory. When removing the tampon, you should exclude its contact with the walls of the vagina.
In the presence of bartholinitis, puncture of the bartholin glands is performed . The contents of the abscess are removed with a sterile swab. When taking material from the uterus, a special tool is used as a syringe-aspirator. In the inflammatory process in the uterine appendages, a smear is taken during surgery or using the method of diagnostic puncture through the vaginal arches.
The results of antibioticograms are determined by units of minimally inhibitory concentration (MIC). To achieve the effect of therapy, it is necessary that the concentration of the drug in the patient’s blood exceed the MIC by approximately four or eight times.