Today is the tank. Sowing is an indispensable analysis for venereologists, urologists, gynecologists, dermatologists and other specialists. This study helps not only determine the causative agent of the disease, but also to find out its quantity and drugs to which it is sensitive. This information is necessary in order to prescribe an effective treatment.
The test material is placed in a laboratory in a favorable environment that promotes the growth of microorganisms. The temperature conditions are very important here. Then the doctor examines the bacteria grown in a microscope and identifies them. In addition, the degree of growth of the pathogen in CFU / ml is necessarily determined.
Next, the sensitivity of the seeded microorganisms to antibiotics is established. The fact is that bacterial resistance to drugs has become a serious problem today. This leads to their uncontrolled reception. Therefore, an antibioticogram is so important, which allows the doctor to choose an effective drug from the entire spectrum of drugs.
Today a tank is being held. inoculation from the cervical canal, vagina, urethra, eyes, ear, nose, as well as urine, saliva, bile, prostate juice, blood, feces, cerebrospinal fluid and pleural fluid. The main advantage of the method is its high accuracy. However, its serious drawback is the execution time, which can reach a week.
This makes it impossible to use the method when treatment must be prescribed quickly, for example, in some acute inflammatory processes. So with acute cystitis and pyelonephritis tank. urine culture usually does not. Although sometimes it is nevertheless performed to clarify the treatment regimen after the result is ready. However, it must be borne in mind that the material must be taken before therapy.
In general, high requirements for patient preparation, collection, transportation and analysis of material are a disadvantage of the method. Violations at any stage can lead to serious distortion of the results.
Despite the use in many areas of medicine, the most widely used this study has been in venereology, gynecology and urology. Most often, a tank is prescribed by doctors. sowing on ureaplasma and mycoplasma, as well as chlamydia, Trichomonas and gonococci. Although to identify the last three pathogens today, PCR is increasingly used. The undoubted advantage of the method is the speed of execution with high sensitivity.
To detect mycoplasmas and ureaplasmas, a tank is more relevant. seeding because they can normally be present in the body. Therefore, it is important not so much their presence, which may be a variant of the norm, as the quantity. These microorganisms cause a pathological process only with rapid growth and large numbers.
Highly sensitive PCR even detects several DNA pathogens in the preparation. Moreover, it can give a positive result even in the presence of microorganisms already killed with antibiotics, but still present for some time in the body. This often occurs when monitoring treatment and is excluded when using seeding.
Gynecologists quite often prescribe a tank. sowing on flora to assess the condition of the reproductive system. Usually a smear for this is taken from the vagina, but sometimes also from the cervical canal and urethra. These three points are close and usually have similar flora and problems. This study helps determine the causative agent of nonspecific colpitis, as well as cervicitis and urethritis.
So tank. Inoculation helps the doctor identify the pathogen, evaluate its activity and sensitivity to drugs, and prescribe effective treatment. This study is used in various fields of medicine, but it has found the widest application in venereology, urology and gynecology.